Rec. PROTEIN GI#
His tag on the recombinant protein
no His tag
Recombinants or rec. proteins
This product is for research use only.
platelet-derived growth factor beta polypeptide
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Other name of the recombinant
SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT
Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/mL.
The lyophilized PDGF-BB recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted PDGF-BB is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing PDGF-BB recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
About recombinant protein
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-β. PDGFR-a is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids). .