Short info
Price
180 EUR
Size
0.1 mg
Catalog no.:
XP-5255
We guarantee fast shipment of Pdgf-BB products. Our specialists will be happy to answer all of your questions.
Product description
NCBI GENE nr
5155
Works with
Human
GENE symbol
PDGFB
Omim nr
129724
Ab raised in
Rabbit
Protein nr
P01127
Ncbi nr
P01127
French translation
anticorps
Type
Polyclonal
Vial with antibody
Lyophilized
Source
Homo sapiens
Antibodie's label
unlabelled serum
Immuno application
ELISA, Western Blot, NELISAut
Antigene name
platelet-derived growth factor beta polypeptide
Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB.
Gene names
SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
Purity
Anti-hPDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Keep at
PDGF-BB antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Antibody advice
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (10.0 ng/mL), a concentration of 0.05 - 0.07 ug/mL of this antibody is required. ELISA:To detect hPDGF-BB by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-BB. Sandwich:To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human PDGF-BB (XP-5255Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.Western Blot:To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.