NCBI GENE nr
Ab raised in
Vial with antibody
ELISA, Western Blot, NELISAut
platelet-derived growth factor beta polypeptide
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPDGF-BB.
SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
Anti-hPDGF-BB specific antibody was purified by affinity chromatography employing immobilized hPDGF-BB matrix.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
PDGF-BB antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (10.0 ng/mL), a concentration of 0.05 - 0.07 ug/mL of this antibody is required. ELISA:To detect hPDGF-BB by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-BB. Sandwich:To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human PDGF-BB (XP-5255Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPDGF-BB.Western Blot:To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.