Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit

The best quality, top Pdgf-BB products prices, quick delivery.

Short info

Price

759 EUR

Size

96 Tests

Catalog no.:

EKE61670

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Product description

Precision

NA

Stability

NA

Specificity

NA

Target Name

NA

Species Reactivity

Mouse

Target's alterntive name

PDGF-BB

Assay Type

Sandwich

Assay Time

4.5 hours

Shipping Conditions

Ice packs

Sensitivity

23.44 pg/mL

Latin name

Mus musculus

Detection Method

Colorimetric

Detection Range

39.06-2500 pg/mL

Estimated Turnaround Time

6-11 business days

Sample Type

Serum, Plasma, Biological Fluids

Storage Temperature

Short term: 4°C; Long term: see manual.

Application

For research use only. Not for diagnostic procedures.

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Cross Activity

No significant cross-reactivity or interference was observed.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Research Area

Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction

Shelf life

Use Mouse PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit before 6 months

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.

Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PDGF-BB . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PDGF-BB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PDGF-BB. The concentration of Mouse PDGF-BB in samples can be calculated by comparing the OD of the samples to the standard curve.