Human PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit

The best Pdgf-BB products, the best prices, high quality.

Short info

Price

759 EUR

Size

96 Tests

Catalog no.:

EKE60948

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Product description

Stability

NA

Precision

NA

Specificity

NA

Target Name

NA

Species Reactivity

Human

Target's alterntive name

PDGF-BB

Assay Type

Sandwich

Shipping Conditions

Ice packs

Assay Time

4.5 hours

Sensitivity

18.75 pg/mL

Detection Method

Colorimetric

Detection Range

31.25-2000 pg/mL

Estimated Turnaround Time

6-11 business days

Sample Type

Serum, Plasma, Biological Fluids

Storage Temperature

Short term: 4°C; Long term: see manual.

Application

For research use only. Not for diagnostic procedures.

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Cross Activity

No significant cross-reactivity or interference was observed.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Research Area

Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction

Shelf life

Use Human PDGF-BB (Platelet Derived Growth Factor BB) ELISA Kit before 6 months

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.Platelets, also called thrombocytes or cloth cells in blood and are needed to stop bleeding by clumping and clotting the blood the vessels when the an injury occurs. Teh bone marrow will produce the platelets that have no nucleus. Platelates are unique to mammals, the are curved shaped 1900nm to 3100 nm large nucleus free clothing structures.

Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-BB . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-BB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDGF-BB. The concentration of Human PDGF-BB in samples can be calculated by comparing the OD of the samples to the standard curve.