Human CellExp PDGF-BB, human recombinant

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Short info

Price

490 EUR

Size

10 μg

Catalog no.:

7276-10

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Product description

Results

N/A

Recombinant

Yes

Purity

N/A

Concentration

N/A

NCBI Gene ID #.

5155

Biomolecule/Target

PDGFB

NCBI Gene Symbol

PDGFB

Gene Source

Human

Accession #

P01127

Shelf Life

1 year

Storage Temp.

-20°C

Shipping

Gel Pack

Group

recombinants

Source

HEK 293 cells

Appearance

Lyophilized powder

Endotoxin Level

<0.1 ng/μg of protein (

Additional source

Recombinants or rec. proteins

Handling

Centrifuge the vial prior to opening.

Alternates names

PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2

Synonyms

PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2

Taglines

Mitogenic during early developmental stages

Usage

For Research Use Only! Not to be used in humans

Biological activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml

Activity (Specifications/test method)

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml

Physical form description

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Molecular Weight

This protein has a calculated MW of 25 kDa. DTT-reduced protein migrates as a 15 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 30 kDa protein.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.