Human CellExp™ PDGF-BB, human recombinant

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Short info

Price

403 EUR

Size

10 μg

Catalog no.:

7276-10

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Product description

Concentration

N/A

Aa sequence

N/A

Recombinant

Yes

Unit

N/A

Binding ability

N/A

Reesults

N/A

NCBI gene number

5155

Target

PDGFB

NCBI gene

PDGFB

Gene source

Human

NCBI number

P01127

Purification

≥95%

Storage condition

-20°C

Shipping under

Gel Pack

Group

recombinants

Tested applications

SDS-PAGE, N/A

Source

HEK 293 cells

Physical appearance

Lyophilized powder

Level of endotoxin

<0.1 ng/μg of protein (

Additional source

Recombinants or rec. proteins

Before use

Centrifuge the vial prior to opening.

Other name

PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2

Synonym

PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2

Notes

For Research Use Only! Not to be used in humans

Biological activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml

Tested activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml

Physical properties

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Molecular weight

This protein has a calculated MW of 25 kDa. DTT-reduced protein migrates as a 15 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 30 kDa protein.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background

PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialized mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.