Short info
Price
490 EUR
Size
10 μg
Catalog no.:
7276-10
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Product description
Results
N/A
Recombinant
Yes
Purity
N/A
Concentration
N/A
NCBI Gene ID #.
5155
Biomolecule/Target
PDGFB
NCBI Gene Symbol
PDGFB
Gene Source
Human
Accession #
P01127
Shelf Life
1 year
Storage Temp.
-20°C
Shipping
Gel Pack
Group
recombinants
Source
HEK 293 cells
Appearance
Lyophilized powder
Endotoxin Level
< 0.1 ng/μg of protein (
Additional source
Recombinants or rec. proteins
Handling
Centrifuge the vial prior to opening.
Alternates names
PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2
Synonyms
PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2
Taglines
Mitogenic during early developmental stages
Usage
For Research Use Only! Not to be used in humans
Biological activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml
Activity (Specifications/test method)
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml
Physical form description
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight
This protein has a calculated MW of 25 kDa. DTT-reduced protein migrates as a 15 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 30 kDa protein.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.