Short info
Price
403 EUR
Size
10 μg
Catalog no.:
7276-10
We guarantee fast shipment of Pdgf-BB products. Our specialists will be happy to answer all of your questions.
Product description
Concentration
N/A
Aa sequence
N/A
Recombinant
Yes
Unit
N/A
Binding ability
N/A
Reesults
N/A
NCBI gene number
5155
Target
PDGFB
NCBI gene
PDGFB
Gene source
Human
NCBI number
P01127
Purification
≥95%
Storage condition
-20°C
Shipping under
Gel Pack
Group
recombinants
Tested applications
SDS-PAGE, N/A
Source
HEK 293 cells
Physical appearance
Lyophilized powder
Level of endotoxin
< 0.1 ng/μg of protein (
Additional source
Recombinants or rec. proteins
Before use
Centrifuge the vial prior to opening.
Other name
PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2
Synonym
PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2
Notes
For Research Use Only! Not to be used in humans
Biological activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml
Tested activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml
Physical properties
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular weight
This protein has a calculated MW of 25 kDa. DTT-reduced protein migrates as a 15 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 30 kDa protein.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Background
PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialized mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.